OBSintermediate
Match each malaria diagnostic method with its defining mechanism or feature: Diagnostic Methods: 1. Light microscopy 2. Rapid diagnostic test (RDT) 3. Polymerase chain reaction (PCR) 4. Placental histology Features: A. Immunochromatographic detection of circulating parasite antigens B. Visual identification of parasites on stained thick and thin blood films C. Microscopic evaluation of tissue sections for sequestered parasites and hemozoin D. Highly sensitive amplification and detection of parasite-specific nucleic acids
Answer
1-B, 2-A, 3-D, 4-C
Explanation
Each approach detects infection in a different way: microscopy visualizes parasites, RDTs detect antigens, PCR detects parasite nucleic acid, and histology examines placental tissue. **Key takeaway:** The method selected depends on clinical setting and diagnostic question.\n\n**Glossary:** **Immunochromatography**: a rapid strip-based antigen detection method; **molecular detection**: identification of genetic material.
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